Figure 1.

Targeted deletion of Traf7 gene causes embryonic lethality

(A) Genotyping analysis of offspring from mating between Traf7^fl/fl;E2a-Cre⁺ (Traf7^GLOhet) mice. Traf7^GLOko mice were never born, but embryonic genotypes (WT and GLOhet) showed the expected Mendelian distribution at E9.5 and E10.5. (dpc): days post-coitus.

(B) Morphological analysis of littermate E9.5 WT and Traf7^GLOko embryos in lateral views inside the intact yolk sac (top panels) and without it (middle panels). Comparison of littermate E10.5 WT and Traf7^GLOko embryos (bottom panel). White scale bars are 0.5 mm.

(C) Analysis of vascular structures in whole mount embryos. Littermate WT and Traf7^GLOko embryos at E9.5 and E10.5 were fixed and immuno-stained with an anti-PECAM-1 mAb (brown). Magnified insets show details of intersomitic vasculature. Black scale bars are 0.5 mm.

(D) Computation of intersomitic vasculature in E10.5 Traf7^GLOko embryos. Each closed symbol represents an individual embryo. Bars show the Median. Asterisks indicate significant difference: (∗) p < 0.05; (∗∗) p < 0.005; (∗∗∗) p < 0.0005; (ns) not significant.