Figure 7.

TRAF7 and SCRIB are essential for phosphorylation of ERK5 in endothelial cells exposed to shear stress

(A) Western blot analysis of ERK5 phosphorylation (p-ERK5) and TRAF7, SCRIB, MEKK3, MEKK2, KLF2, and KLF4 protein expression in HUVECs under normal (−) or shear stress (+) conditions, with addition of esiRNA specifically inhibiting each of the proteins as indicated. Anti-Renilla luciferase (RLUC) esiRNA was used as a negative control. Anti-α-Tubulin antibody was used as the loading control. The results are representative of least three independent experiments.

(B) Protein gel quantification analysis of the top western blot panel in (A). p-ERK5 protein levels were calculated as percent of total ERK5 protein content in corresponding lanes using GeneTools from Syngene software. Western blots from four independent experiments were analyzed. Data are presented as Mean ± StDev.