REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal ANTI-FLAG® M2 antibody produced in mouse	Sigma-Aldrich	Cat#F1804; RRID: AB_262044
Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate	BIO-RAD	1705047; RRID:AB_11125753
Anti-CD31, rat monoclonal (MEC13.3) anti-mouse	Fisher Scientific	BDB557355
Goat anti-Rat IgG, polyclonal Affinity-Purified secondary Antibody, HRP	Fisher Scientific	OB3010-05
HA-Tag (C29F4) Rabbit mAb	Cell Signaling Technology	Cat#3724; RRID:AB_1549585
Anti-TRAF7 (WD40) antibody produced in rabbit	Sigma-Aldrich	Cat#HPA041229; RRID:AB_10794194
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Invitrogen)	Fisher Scientific	Cat#31462; RRID:AB_228338
MEKK3 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen	Fisher Scientific	Cat#PIPA578058
MEKK2, Rabbit polyclonal	Cell Signaling Technology	Cat#19607; RRID:AB_2798822
MEK5 (E4C4B) Rabbit monoclonal	Cell Signaling Technology	Cat#40737
SCRIB, Rabbit polyclonal	Cell Signaling Technology	Cat#4475; RRID:AB_10557101
Anti-KLF4, rabbit polyclonal	Sigma-Aldrich	Cat#SAB2107958
KLF2 Rabbit polyclonal, Invitrogen™	Fisher Scientific	Cat#PIPA540591
α-Tubulin (DM1A) Mouse monoclonal	Cell Signaling Technology	Cat#3873; RRID:AB_1904178
Anti-Sp1 antibody, Rabbit polyclonal	Sigma-Aldrich	Cat#07645; RRID:AB_310773
Chemicals, peptides, and recombinant proteins
Tamoxifen	MP Biomedicals	Cat#215673883
TrypLE™ Express Enzyme	Fisher Scientific	Cat#12605010
Critical commercial assays
RNeasy Mini Plus Kit	QIAGEN	Cat#74136
QIAshredder	QIAGEN	Cat#79656
RNAlater® Solution	Fisher Scientific	Cat#AM7023
Lipofectamine™ LTX Reagent	Invitrogen	Cat#15338100
Lipofectamine™ 3000 Transfection Reagent	Invitrogen	Cat#L3000015
qScript™ XLT One-Step RT-qPCR ToughMix®, Low ROX™	QuantaBio	Cat#95134
PerfeCTa® qPCR FastMix® II, Low ROX™	QuantaBio	Cat#95120
Phusion Site-Directed Mutagenesis Kit	Thermo Scientific	Cat#F-541
Deposited data
RNA-seq raw data files	NCBI Gene Expression Omnibus	GEO: GSE229698
Experimental models: Cell lines
Human Umbilical Vein Endothelial Cells (HUVEC)	Fisher Scientific	Cat#C0035C; RRID:CVCL_2959
293 human embryonic kidney cell line	Sigma-Aldrich	Cat#85120602
COS-1 African green monkey kidney cell line	Sigma-Aldrich	Cat#88031701
Experimental models: Organisms/strains
Mouse: Traf7+/fl on C57BL/6 background	This paper	N/A
Mouse: B6.FVB-Tg(EIIa-cre)C5379Lmgd/J	The Jackson Laboratory	JAX: 003724; RRID:IMSR_JAX:003724
Mouse: B6.Cg-Tg(Tek-cre)12Flv/J	The Jackson Laboratory	JAX: 004128; RRID:IMSR_JAX:004128
Mouse: C57BL/6-Tg(Cdh5-cre/ERT2)1Rha	Taconic	Model: 13073
Oligonucleotides
MISSION® esiRNA targeting human TRAF7	Sigma-Aldrich	EHU071571-20UG
MISSION® esiRNA targeting human SCRIB	Sigma-Aldrich	EHU141131-20UG
MISSION® esiRNA targeting human MAP3K3	Sigma-Aldrich	EHU101361-20UG
MISSION® esiRNA targeting human MAP2K3	Sigma-Aldrich	EHU088281-20UG
MISSION® esiRNA targeting RLUC	Sigma-Aldrich	EHURLUC-20UG
Primers for Traf7 mice genotyping by conventional PCR (PCR product: WT allele 242 bp, floxed allele 375 bp): T7-Fwd: GCCATAGGGACATTTAACCTGCA T7-Rev: CTAATGCCATACTGCAGACCCA	This paper	N/A
TaqMan assay for genotyping of the WT Traf7 mouse allele by real-time PCR: WT-3’-Fwd: CCTTGGGCTTAACTCTGCTTAT WT-3’-Rev: CACCCAGAAAGTCCAACAAGA WT-3’-Probe: CAGCCTTGGGAAGAGCCTACTGAC	This paper	N/A
TaqMan assay for genotyping of the floxed Traf7 mouse allele by real-time PCR: Flox-3’-Fwd: GCTAGTACCTTGGGCTTAACTC Flox-3’-Rev: TGTTCGAACGAAGTTCCTATTCT Flox-3’-Probe: ACGGACACAATCCCACGAACGTAC	This paper	N/A
TaqMan assay for genotyping of the knockout Traf7 mouse allele by real-time PCR: KO-Fwd: GAGCTAGTTGGCTTCCTCAAA KO-Rev : GGGAAGAGCCTACTGACAGATTA KO-Probe: TGGTACCGTACGTGTAGAAGCTACCA	This paper	N/A
TaqMan assay for mouse GAPDH RT-qPCR: GAPDH-Fwd: CCTGTTGCTGTAGCCGTATT GAPDH-Rev: AACAGCAACTCCCACTCTTC GAPDH-Probe: TTGTCATTGAGAGCAATGCCAGCC	This paper	N/A
TaqMan assay for mouse KLF2 RT-qPCR: KLF2-Fwd: GGCAAGACCTACACCAAGAG KLF2-Rev: TCCTTCCCAGTTGCAATGAT KLF2-Probe: CGTACACACACAGGTGAGAAGCCT	This paper	N/A
TaqMan assay for mouse KLF4 RT-qPCR: KLF4-Fwd: TTTCCTGCCAGACCAGATG KLF4-Rev: CTTTGGCTTGGGCTCCT KLF4-Probe: TTATCAAGAGCTCATGCCACCGGG	This paper	N/A
TaqMan assay for mouse PECAM1 RT-qPCR: PECAM1-Fwd: GTGGTCATCGCCACCTTAATA PECAM1-Rev: TTCTCGCTGTTGGAGTTCAG PECAM1-Probe: AAAGCCAAGGCCAAACAGAAACCC	This paper	N/A
Recombinant DNA
p3XFLAG-CMV™-7.1 Expression Vector	Sigma-Aldrich	Cat#E7533
p3XFLAG-CMV™-7-BAP Control Plasmid	Sigma-Aldrich	Cat#C7472
Human TRAF7 (NM_032271) ORF Clone in pcDNA3.1+/C-(K)DYK	GenScript	Cat#OHu30808D
SCRIB (H. sapiens) in pANT7_cGST	DNASU Plasmid Repository	CloneID: HsCD00731633
RFWD2 (H. sapiens) in pANT7_cGST	DNASU Plasmid Repository	CloneID: HsCD00639431
pCMV-HA	Clontech	Cat#631604
mCh-Climp63	AddGene	Cat#136293; RRID:Addgene_136293
pD2EGFP-N1	This paper	N/A
p3XFLAG-CMV™-7.1-COP1	This paper	N/A
p3XFLAG-CMV™-7.1-TRAF7	This paper	N/A
p3XFLAG-CMV™-7.1-TRAF7-N520S	This paper	N/A
p3XFLAG-CMV™-7.1-TRAF7 (1-670)	This paper	N/A
p3XFLAG-CMV™-7.1-TRAF7 (1-384)	This paper	N/A
p3XFLAG-CMV™-7.1-TRAF7 (1-292)	This paper	N/A
p3XFLAG-CMV™-7.1-TRAF7 (1-127)	This paper	N/A
p3XFLAG-CMV™-7.1-TRAF7 (121-292)	This paper	N/A
p3XFLAG-CMV™-7.1-TRAF7 (281-670)	This paper	N/A
p3XFLAG-CMV™-7.1-TRAF7 (375-670)	This paper	N/A
Software and algorithms
GraphPad Prism 9	GraphPad Software	Prism - GraphPad
HISAT2 (v2.0.5)	SciCrunch Registry	RRID:SCR_015530
featureCounts (v1.5.0-p3)	SciCrunch Registry	RRID:SCR_012919
DESeq2 R package (v1.20.0)	SciCrunch Registry	RRID:SCR_012823
edgeR (v3.22.5)	SciCrunch Registry	RRID:SCR_012802
GATK (v4.1.1.0)	SciCrunch Registry	RRID:SCR_001876
rMATS (4.1.0)	SciCrunch Registry	RRID:SCR_023485
STAR-Fusion (v1.9.0)	Novogene	https://www.novogene.com/
clusterProfiler	SciCrunch Registry	RRID:SCR_016884
STAR	SciCrunch Registry	RRID:SCR_004463