Figure 5.

Washout experiments indicate low reversibility of PMX and egress inhibition mediated by macrocycle 7k. (A) Washout of the macrocycle inhibitors allows restoration of SUB1 maturation in the case of compounds 7a and 7j but not in the case of 7k. (B) Washout of the macrocycle inhibitors allows restoration of parasite egress (schizont rupture) and new ring formation in the case of compounds 7a and 7j but not in the case of 7k. The plot shows both new ring formation and levels of residual unruptured schizonts after 3.5 h of further culture following washout.