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. 2023 Jul 12;27(16):2412–2423. doi: 10.1111/jcmm.17827

FIGURE 3.

FIGURE 3

AMIGO2 reduced innate cisplatin sensitivity via suppression of GSDME‐mediated pyroptosis. (A) A549 cells simultaneously transfected with AMIGO2 siRNA and GSDME siRNA were treated with 5 μg/mL cisplatin for 48 h prior to detection of cell viability by MTT method. (B) A549 cells simultaneously transfected with AMIGO2 siRNA and GSDME siRNA were treated with 5 μg/mL cisplatin for 24 h, and subjected to western blot analysis with the indicated antibodies. (C) A549 cells simultaneously transfected with AMIGO2 siRNA and GSDME siRNA were treated with 5 μg/mL cisplatin for 24 h, and dual stained with 2 μg/mL PI and 5 μg/mL Hoechst 33342 for 10 min in dark. After capturing the images by fluorescence microscopy (20× objective lens), PI‐positive cells were calculated in five random fields and statistically analysed. (D) The percentage of LDH release in the culture supernatants from cells treated with cisplatin for 24 h was measured using LDH cytotoxicity assay kit. *p < 0.05; **p < 0.01.