FIGURE 5.

AMIGO2 inhibited pyroptosis through activation of PDK1/Akt (T308) signalling pathway. (A) Proteins extracted from A549/shAMIGO2 and H460/AMIGO2 cells were subjected to western blot analysis with the indicated antibodies. (B–D) H460/AMIGO2 cells pretreated with BX795 (5 μM) or MK‐2206 (1 μM) for 4 h were stimulated with 3 μg/mL cisplatin for 24 h. (B) Proteins in cell lysates were analysed by western blotting with the indicated antibodies. (C) MTT method was performed to assess cell viability. (D) The LDH release in the culture supernatants from cells was assessed using LDH cytotoxicity assay kit. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant.