FIGURE 6.

Knockdown of AMIGO2 increased cisplatin sensitivity in vivo. A549/shAMIGO2 or A549/shNC cells (3 × 10⁶) mixed with Matrigel were subcutaneously injected to the right flank of four‐to‐five‐week‐old male BALB/c nude mice. When tumours formed, mice were randomly divided into 4 groups as described in Materials and Methods. The mice received intraperitoneal injections of cisplatin at a dose of 3 mg/kg every 3 days, whereas the control group received PBS. (A) Tumour growth curves were determined by calculation of the tumour volume every 3 days. (B) Photographs of the tumours extirpated from the engrafted mice. (C) Comparison of tumour weights of the indicated groups. (D) Body weights were measured once every 3 days. (E) Tumours were lysed and subjected to western blot analysis of the cleavage of GSDME. GAPDH was used as the loading control. (F) The supernatants from tumour tissue lysate were collected for measurement of the LDH activity using Lactate dehydrogenase assay kit according to the manufacturer's instructions. (G) Tumour sections were immunostained with anti‐Ki67 and anti‐CD34 antibodies. (H, I) Image‐Pro® Plus v 6.0 (for Windows) was used to calculate the integrated optical density (IOD) per unit (Area) of the images. *p < 0.05; **p < 0.01; ns, not significant.