FIG. 5.

Analysis of transcripts generated in the presence or absence of SAM. (A) Northern blot analysis. Total RNA synthesized in the presence or absence of SAM was probed essentially as described in the legend to Fig. 3. (B) Immunoprecipitation analysis. Total RNA synthesized in the presence or absence of SAM was hybridized to end-labeled oligonucleotides complementary to regions in the GFP mRNA (nucleotides 73 to 94) and CAT mRNA (nucleotides 56 to 75). Following extension, the RNA-cDNA hybrids were immunoprecipitated essentially as described in the legend to Fig. 4. WT, wild type.