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. Author manuscript; available in PMC: 2023 Aug 14.
Published in final edited form as: Bioconjug Chem. 2022 Oct 4;33(10):1803–1810. doi: 10.1021/acs.bioconjchem.2c00389

Figure 2. In vitro BBB Analysis Demonstrates Specificity of Transferrin Aptamer for Receptor.

Figure 2.

(A) Representation of ProSNAs crossing an in vitro BBB model. Cells were treated with fluorescently labeled proteins on the apical side of a transwell insert. (B) Normalized fluorescence readings of the basolateral side were taken periodically and plotted to measure the protein’s transcytosis efficiency. Each point represents the mean of n =4 measurements, the error bars show SD, and the line is a nonlinear regression fit graphed using GraphPad Prism. (C) Explicit fluorescence significance comparison after 90 minutes incubation of either Dextran 70 kDa, β-Gal-AF647, TfR ProSNA or Scr ProSNA. The statistical significance was analyzed using ordinary one-way ANOVA, followed by tukey multiple comparison. **** p-value < 0.0001, *** p < 0.001, ** p < 0.01, and * p < 0.05.