Scheme of metabolic pathway in T. brucei bloodstream forms. Rectangles indicate steps of glucose and threonine metabolism; dashed arrows indicate steps for which no evidence of flux is available. A, ATPase; AcCoA, acetyl-CoA; FAS II, type II fatty-acid biosynthesis pathway; DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde 3-phosphate; G3P, glycerol 3-phosphate; GPDH, glycerol 3-phosphate dehydrogenase; PEP, phosphoenolpyruvate; Pyr, pyruvate; UQ, ubiquinone; TAO, trypanosome alternative oxidase. Enzymes are: 1. Pyruvate dehydrogenase; 7, threonine dehydrogenase. Activity stimulated by Ca2+ is in yellow. Figure 2. Phylogenetic tree of trypanosomatid and human MCU complex subunits. Thee scale bar corresponds to a distance of 20 changes per 100 amino acid positions. Reproduced with permission from reference (Huang et al., 2013b).