Figure 3.

A. Scheme depicting the putative organization and composition of a hetero-hexameric TbMCU complex. Reproduced with permission from reference (Huang, Docampo, 2018). B. conserved WDXXEPXTY motif in TcMCU complex subunits. Alignment of the C-terminal fragment of the first transmembrane domain (TM1, in blue) and the N-terminal fragment of the second transmembrane domain (TM2, in green), including conserved WDXXEPXTY motif (in red), of TcMCU complex subunits with human MCU and MCUb. Conserved putative critical acidic residues in or near the WDXXEPXTY selectivity filter are indicated. TcMCU, TcMCUb, TcMCUc and TcMCUd exhibit a substitution of the Ru360-sensitive residue S to D/G in the pore region. Residues in each TcMCU complex subunit that were subjected to substitutions are indicated with the corresponding number in their sequence. Reproduced with permission from reference (Chiurillo et al., 2019).