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16S rDNA hypervariable regions sequenced in microbiome projects.
| Hypervariable regions | Sample types | Primer pairs |
|---|---|---|
| V1–V2 | Activated sludge 64 | 27f-338r, 8f/357r |
| V1–V3 | Freshwater 65 | 8f/556r |
| V3–V4 | Freshwater55,66 | 341f/805r, 341f/785r |
| V4 | Freshwater 67 | 515f/806r, 515f/808r |
| V4–V5 | Anaerobic digester10,68 | 515f/909r, 563F/926R |
| V5–V6 | Freshwater3,69, Anaerobic digester 33 | 807f/1050r |
| V6–V8 | Anaerobic digester 70 | 926f/1392r |
