Figure 7. BCR signaling is a prerequisite to compete in the LZ.

(A) Distribution of B1-8^hi BTK^C481S and B1-8^hi BTK^WT from mice treated with vehicle alone (far-left, left) or with acalabrutinib (right, far-right). Number of cells in condition denoted. (B) Cluster distribution of B1-8^hi BTK^C481S and B1-8^hi BTK^WT from mice treated with vehicle (left) and acalabrutinib (right). Frequencies calculated from a random sample of 100 cells from each population. (C) Enrichment of signature upregulated in WT vs BTK KO cells, visualized by signatures scores⁵⁶. (D) NP (left) and Y-Ae (right) MFIs visualized on UMAP. Gray circles mark NP⁻ (left, MFIs <1000) and Y-Ae⁻ (right, MFIs <150) cells. (E) Cluster distribution of B1-8^hi BTK^C481S and B1-8^hi BTK^WT NP-Eα⁺ cells from mice treated with vehicle (left) or with acalabrutinib (right). Frequencies calculated from a random sample of 85 cells from each population. Autocorrelation and p value depicted on graph (C).