REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat Anti-Mouse Anti-CD35	Absolute Antibody	Cat#: Ab00238-7.1, RRID:AB_397114
PE Rabbit Anti-Mouse Active Caspase 3, Clone: C92 605	BD Biosciences	Cat# 550821, RRID:AB_393906
PE Rat Anti-Mouse CD184, Clone 2B11	BD Biosciences	Cat# 551966, RRID:AB_394305
Rat Anti-Mouse CD16/CD32 , Clone 2.4G2	BD Biosciences	Cat# 553142, RRID:AB_394657
PE-Cy7 Hamster Anti-Mouse CD95, Clone Jo2	BD Biosciences	Cat# 557653, RRID:AB_396768
V450 Rabbit Anti-Mouse Active Caspase-3, Clone C92-605	BD Biosciences	Cat# 560627, RRID:AB_1727415
BV421 Hamster Anti-Mouse CD95, Clone Jo2	BD Biosciences	Cat# 562633, RRID:AB_2737690
BV421 Rat Anti-Mouse CD184, Clone 2B11	BD Biosciences	Cat# 562738, RRID:AB_2737757
BV605 Rat Anti-Mouse CD45R/B220, Clone RA3-6B2	BD Biosciences	Cat# 563708, RRID:AB_2738383
BV421Mouse Anti-Mouse CD45.1, Clone A20	BD Biosciences	Cat# 563983, RRID:AB_2738523
BV480 Streptavidin	BD Biosciences	Cat# 564876, RRID:AB_2869619
BV480 Rat Anti-Mouse CD45R/B220, Clone RA3-6B2	BD Biosciences	Cat# 565631, RRID:AB_2739311
BV421 Rat Anti-Mouse CD205 (DEC-205), Clone V18-949	BD Biosciences	Cat# 566375, RRID:AB_2744323
BUV737 Anti-Mouse CD45.2, Clone 104	BD Biosciences	Cat# 612778, RRID:AB_2870107
BUV737 Mouse Anti-Mouse CD45.1, Clone A20	BD Biosciences	Cat# 612811, RRID:AB_2870136
BV711 Rat Anti-Mouse CD86, Clone GL1	BD Biosciences	Cat# 740688, RRID:AB_2734766
BUV496 Anti-Mouse CD38, Clone 90	BD Biosciences	Cat# 741090, RRID:AB_2916913
BUV395 Rat Anti-Mouse Ig, λ1, λ2 & λ3 Light Chain, Clone R26-46	BD Biosciences	Cat# 744529, RRID:AB_2742303
BV480 Mouse Anti-Mouse CD45.1, Clone A20	BD Biosciences	Cat# 746666, RRID:AB_2743938
BUV805 Rat Anti-Mouse CD45R/B220, Clone RA3-6B2	BD Biosciences	Cat# 748867, RRID:AB_2873270
HuCAL Fab-MH NEGATIVE CONTROL	Bio-Rad	Cat# HCA051, RRID:AB_915480
Alexa Fluor 488 Rat Anti-Mouse CD38, Clone 90	BioLegend	Cat# 102714, RRID:AB_528796
FITC Mouse Anti-Mouse CD45.2, Clone 104	BioLegend	Cat# 109806, RRID:AB_313443
APC Mouse Anti-Mouse CD45.2, Clone 104	BioLegend	Cat# 109814, RRID:AB_389211
Alexa Fluor 488 Mouse Anti-Mouse CD45.2, Clone 104	BioLegend	Cat# 109816, RRID:AB_492868
Brilliant Violet 785 Mouse Anti-CD45.2 Mouse, Clone: 104	BioLegend	Cat# 109839, RRID:AB_2562604
PE Mouse Anti-Mouse CD45.1, Clone A20	BioLegend	Cat# 110708, RRID:AB_313497
Alexa Fluor 700 Mouse Anti-Mouse CD45.1, Clone: A20	BioLegend	Cat# 110724, RRID:AB_493733
APC Rat Anti-Mouse CD205, Clone: NLDC-145	BioLegend	Cat# 138206, RRID:AB_10613641
Biotin-SP (long spacer) AffiniPure Goat Anti-Mouse IgM, μ chain specific	Jackson Immunoresearch	Code: 115-065-020, RRID: AB_2338560
Recombinant αDEC-OVA	Produced In house	N/A
Recombinant αDEC-CS	Produced In house	N/A
Recombinant αDEC-OVA-Eα	Produced In house	N/A
PE Rat Anti-Mouse CCR6 Monoclonal antibody, Clone 140706	R and D Systems	Cat# FAB590P, RRID:AB_2244251
Biotin Mouse Anti-Mouse Ea52-68 peptide bound to I-A Mouse, Clone Yae	Thermo Fisher Scientific	Cat# 13-5741-85, RRID:AB_657823
APC-eFluor 780, eBioscience Anti-Mouse CD4, Clone RM4-5	Thermo Fisher Scientific	Cat# 47-0042-82, RRID:AB_1272183
APC-eFluor 780, eBioscience Anti-Mouse CD8a, Clone 53-6.7	Thermo Fisher Scientific	Cat# 47-0081-82, RRID:AB_1272185
APC-eFluor 780, eBioscience Anti-Mouse F4/80, Clone BM8	Thermo Fisher Scientific	Cat# 47-4801-82, RRID:AB_2735036
APC-eFluor 780, eBioscience Anti-Mouse Tcr beta, Clone H57-597	Thermo Fisher Scientific	Cat# 47-5961-82, RRID:AB_1272173
Alexa Fluor 700, eBioscience Anti-Mouse CD38, Clone 90	Thermo Fisher Scientific	Cat# 56-0381-82, RRID:AB_657740
Chemicals, peptides, and recombinant proteins
Cytofix/Cytoperm Fixation/Permeabilization Solution Kit	BD Biosciences	Cat# 554714
NP-OVAL (Ovalbumin) Conjugation Ratio 17	Biosearch Technologies	Item ID N-5051-10
NP-OSu	Biosearch Technologies	Item ID N-1010-100
NIP-BSA-Biotin, Conjugation Ratio 16	Biosearch Technologies	Item ID N-1027-5
NP-BSA-Biotin, Conjugation Ratio 2	Biosearch Technologies	Item ID N-1026-5
NP-BSA-Biotin, Conjugation Ratio 9	Biosearch Technologies	Item ID N-1026-5
Alhydrogel® adjuvant 2%	InvivoGen	Cat# vac-alu-250
Acalabrutinib	MedChem Express	Cat# HY-17600
Biotinylated Eα peptide	Nussenzweig Lab	N/A
Buffer TCL	Qiagen	Cat# 1031576
Ibrutinib, Free Base	Selleckchem	Cat# S2680
2-Mercaptoethanol,BioUltra, for molecular biology, ≥99.0% (GC)	Sigma-Aldrich	SKU 63689; CAS 60-24-2
Igepal® CA-630,for molecular biology	Sigma-Aldrich	SKU I8896; CAS 9002-93-1
N,N-Dimethylformamide,anhydrous, 99.8%	Sigma-Aldrich	SKU 227056; CAS 68-12-2
(2-Hydroxypropyl)-beta-cyclodextrin,powder, BioReagent, suitable for cell culture	Sigma-Aldrich	SKU C0926; CAS 128446-35-5
Betaine solution, 5 M, PCR Reagent	Sigma-Aldrich	SKU B0300; CAS 107-43-7
Dimethyl sulfoxide	Sigma-Aldrich	SKU D2438; CAS 67-68-5
Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)	Sigma-Aldrich	SKU A5503; CAS 9006-59-1
Hydrochloric acid solution	Sigma-Aldrich	SKU H9892; CAS 7647-01-0
Bovine Serum Albumin	Sigma-Aldrich	SKU A2153; CAS 9048-46-8
Fetal Bovine Serum	Sigma-Aldrich	SKU F8192; MDL MFCD00132239
ACK Buffer	Thermo Fisher Scientific	Cat# A1049201
RPMI 1640 Medium, no phenol red	Thermo Fisher Scientific	Cat# 11835030
DPBS, no calcium, no magnesium	Thermo Fisher Scientific	Cat# 14190144
Thermo Scientific Imject Alum Adjuvant	Thermo Fisher Scientific	Cat# 77161
RPMI Buffer	Thermo Fisher Scientific	Cat#11875093
Streptavidin, Alexa Fluor™ 647 conjugate	Thermo Fisher Scientific	Cat# S21374
Streptavidin, Alexa Fluor™ 594 conjugate	Thermo Fisher Scientific	Cat# S11227
Streptavidin, Alexa Fluor™ 488 conjugate	Thermo Fisher Scientific	Cat# S11223
Life Technologies Powerload Concentrate 100x	Thermo Fisher Scientific	Cat# P10020
Probenecid, Water Soluble	Thermo Fisher Scientific	Cat# P36400
HEPES	Thermo Fisher Scientific	Cat# 15630080
Indo-1 AM, cell permeant	Thermo Fisher Scientific	Cat# I1223
Powerload Concentrate 100x	Thermo Fisher Scientific	Cat# P10020
D-Biotin	Thermo Fisher Scientific	Cat# B20656
Critical Commercial Reagents
Agencourt RNAClean XP, 40 mL	Beckman Coulter	Product# A63987
Agencourt AMPure XP	Beckman Coulter	Product# A63881
Sheep Red Blood Cells	Colorado Serum Company	Cat# 31112
Ni Sepharose 6 Fast Flow, 100 mL	Cytiva	Product# 17531802
Nextera XT DNA Library Preparation Kit	Illumina	Cat# FC-131-1096
Nextera XT Index Kit v2 Set A	Illumina	Cat# FC-131-2001
Illumina DNA Prep, (M) Tagmentation	Illumina	Cat# 20060059
IDT® for Illumina® DNA/RNA UD Indexes Set A	Illumina	Cat# 20027213
IDT® for Illumina® DNA/RNA UD Indexes Set B	Illumina	Cat# 20027214
IDT® for Illumina® DNA/RNA UD Indexes Set C	Illumina	Cat# 20027215
IDT® for Illumina® DNA/RNA UD Indexes Set D	Illumina	Cat# 20027216
Feeding Tube 20ga 38mm 250 Pk	Instech Laboratories	Part# FTP2038
Streptavidin, Unconjugated	Jackson ImmunoResearch	Code# 016-000-113
CD43 (Ly-48) MicroBeads, mouse	Miltenyi Biotec	Order# 130-049-801
Anti-Ter-119 MicroBeads, mouse	Miltenyi Biotec	Order# 130-049-901
LS Columns	Miltenyi Biotec	Order# 130-042-401
Pre-separation Filters	Miltenyi Biotec	Order# 130-041-407
NEB® 5-alpha Competent E. coli (High Efficiency)	New England Biolabs	Cat# C2987I
Quick Ligation™ Kit	New England Biolabs	Cat# M2200L
Quick CIP	New England Biolabs	Cat# M0525L
AgeI-HF® Restriction Enzyme	New England Biolabs	Cat# R3552L
SalI-HF	New England Biolabs	Cat# R3138L
XhoI	New England Biolabs	Cat# R0146L
QIAquick PCR & Gel Cleanup Kit	Qiagen	Cat# 28506
KAPA HiFi HotStart ReadyMix	Roche	Material# 7958935001
Kinetics Buffer 10X	Sartorius	Item# 18-1105
High precision streptavidin (SAX)	Sartorius	Item# 18-5119
Anti-Human Fab-CH1 (FAB2G)	Sartorius	Item# 18-5127
Pierce™ Slide-A-Lyzer® MINI Dialysis Units, MWCO=10K	Thermo Fisher Scientific	Cat# 88404
Pierce™ Protein Concentrators, MWCO=10 kD	Thermo Fisher Scientific	Cat# 88513
Maxima H Minus Reverse Transcriptase	Thermo Fisher Scientific	Cat# EP0753
Thermo Scientific Adhesive PCR Plate Seals	Thermo Fisher Scientific	Cat# AB-0626
Qubit™ dsDNA HS and BR Assay Kits	Thermo Fisher Scientific	Cat# Q32854
Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits	Thermo Fisher Scientific	Cat# Q33231
Deposited Data
Bulk-RNA seq (GC B1-8hi cells, Myc and NP-Ea)	this paper	GSE225573
sc-RNA seq (LZ B1-8hi BTKC481S and LZ B1-8hi BTKWT)	this paper	GSE225574
Experimental models: Cell Lines
Expi293F	Thermo Fisher Scientific	Cat# A14527, RRID:CVCL_D615
Experimental models: Organisms/strains
B1-8hi	Nussenzweig Lab	N/A
BTKC481S	Nussenzweig Lab	N/A
DEC205−/−	Nussenzweig Lab	N/A
B6;129-Myctm1Slek/J	The Jackson Laboratory	Strain #:021935, RRID:IMSR_JAX:021935
B6.SJL-Ptprca Pepcb/BoyJ	The Jackson Laboratory	Strain #:002014, RRID:IMSR_JAX:002014
C57BL/6-Tg(Nr4a1-EGFP/cre)820Khog/J	The Jackson Laboratory	Strain #:016617, RRID:IMSR_JAX:016617
C57BL/6J	The Jackson Laboratory	Strain #:000664, RRID:IMSR_JAX:000664
Tg(CAG-ECFP)CK6Nagy/J	The Jackson Laboratory	Strain #:003773, RRID:IMSR_JAX:003773
Oligonucleotides
See Table S2
Software and algorithms
Velocyto	Bergen et al.50	http://velocyto.org/
scVelo	Kowalczyk et al.51	https://scvelo.readthedocs.io/
PANDAseq	Masella et al.85	https://github.com/neufeld/pandaseq
kallisto	Bray et al.87	https://pachterlab.github.io/kallisto/about
tximport	Soneson et al.88	https://bioconductor.org/packages/release/bioc/html/tximport.html
DESeq2	Love et al.89	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
Gene Set Enrichment Analysis	Subramanian et al.90	https://www.gsea-msigdb.org/gsea/index.jsp
STARsolo	Kaminow et al.92	https://github.com/alexdobin/STAR
Seurat	Hao et al.93	https://satijalab.org/seurat/
sctransform	Choudhary et al.94	https://satijalab.org/seurat/index.html
TRUST4	Song et al.95	https://github.com/liulab-dfci/TRUST4/releases
VISION	DeTomaso et al.96	https://yoseflab.github.io/VISION/
R	N/A	https://www.r-project.org/
GraphPad Prism	N/A	https://www.graphpad.com/
FlowJo	N/A	https://www.flowjo.com/
Octet® Analysis Studio	N/A	https://www.sartorius.com/en
FastX-toolkit	N/A	http://hannonlab.cshl.edu/fastx_toolkit/
Adobe Illustrator	N/A	https://www.adobe.com/