Extended Data Fig. 1. Fragmentation of Y-chromosome after micronucleation.

(A) Quantitation of chromosomal fragment tethering for RPE1 WT vs p53^−/− cells (similar experimental setup as used in Fig.1C for Mps1i condition) (n=9 independent experiments, total 498 and 861 cells were analyzed for RPE1 WT and RPE1 p53^−/− conditions, respectively). Two-tailed unpaired t-test was applied, P=0.0009. (B) Experimental schematic for (C) and (D). (C) Representative images of Y-chromosome in chromosome spreads from experiment outlined in (B). Yellow arrows point to Y-chromosome fragments. (D) Quantitation of fragmented Y-chromosome from experiments outlined in (B) (n=3 independent experiments, total 203 cells were analyzed). For (A) and (D), mean +/− SEM are shown.