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. Author manuscript; available in PMC: 2024 Jul 17.
Published in final edited form as: Chembiochem. 2023 Jun 21;24(14):e202300073. doi: 10.1002/cbic.202300073

Figure 3. Surfaceome tagging of WT and mutant LX-2 cells.

Figure 3.

(A) Confocal microscopy images of WT LX-2 cells labeled by the surfaceome profiling technique. Biotin signals (red) were mostly located on cell surfaces that surround nuclei (blue). The absence of a labeling component (e.g. lipid, APEX2-LPETG, or eSrtA) resulted in abrogation of biotinylation signals, compared to the full reaction (Rxn). (B) Volcano plots depicting enrichment of surfaceome labeled proteins from WT LX-2 and mutant cell lines over negative controls (conditions lacking APEX2-LPETG). Proteins with ≥2 unique peptides that were significantly enriched (p < 0.05) by the surfaceome capture method and annotated as a cell surface protein (CSP) in UniProt are present in the upper right quadrant. Representative canonical CSPs are highlighted.