Figure 4. Comparison of CSPs in WT and mutant LX-2 cell lines.

(A) Representative volcano plots of relative enrichment of CSPs between surfaceome labeled WT and mutant LX-2 cell lines across biological duplicates. Areas close to the vertical dashed line mark enrichment between cell lines, and the areas above the horizonal dashed line mark p < 0.05. Proteins within the upper right quadrant were significantly enriched in the WT over compared condition. Orange proteins were consistently identified across replicates. The most significantly enriched and prioritized proteins for study are enclosed within a dashed orange box. (B) Average raw TMT abundance values of Bsg and Nptn in their respective cell lines, as well as MCT1 and MCT4 across all mutant cell lines. Bsg and Nptn were enriched significantly in WT cells over respective mutant cell lines. MCT1 and MCT4 were significantly enriched in WT over Bsg^MUT cells, but not over Nptn^MUT cells. Error bars indicate standard deviation. Asterisks indicate statistical significance (p < 0.05) as determined by multiple unpaired t-test.