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293T cell NAMPT overexpression results in higher NAD+ levels and lower growth inhibition in the presence of metformin. (A) Western blot analysis following transfection of NAMPT mRNA or Empty Vector (EV) in 293T cells. (B) Following NAMPT mRNA transfection, 293T cells were treated for 1 day, counted via trypan exclusion assay (*p = 0.023, #p = 0.00060). (C) Growth inhibition analysis via Trypan Blue assay of 293T mRNA transfection followed by metformin treatment (*p = 0.023).
