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. 2023 Jul 31;14:1190707. doi: 10.3389/fimmu.2023.1190707

Figure 1.

Figure 1

Gelsevirine (GS) inhibits cytosolic DNA-induced expression of interferon and cytokines. Chemical structures of GS (A), and dose-dependent inhibitory curves (b, c, n = 3) a fter pretreatment with different concentrations of GS for 6 hrs, Raw264.7 and THP-1 cells were stimulated by 2’3’-cGAMP (5 μg/ml) for 3 hrs. Ifnb1 mRNA expression in Raw264.7 cells (B) and IFNB1 mRNA expression in THP-1 cells (C) were measured by RT-PCR. Raw264.7 and THP-1 cells were pretreated with GS (10 μM) for 6 hrs and then stimulated with 2’3’-cGAMP (5 μg/ml), ISD (2 μg/ml), or Poly(dA:dT) (5 μg/ml) for 3 hrs. The mRNA expression of Cxcl10 (D) and Il6 (F) in Raw264.7 cells and the mRNA expression of CXCL10 (E) and IL6 (G) in THP-1 cells were measured by RT-PCR. *P < 0.05 vs vehicle group.