Table 2.
Statistically significant between-group differences in terms of laboratory biomarkers in controls and remitting- and relapsing-multiple sclerosis patients.
| A - Coagulation/complement biomarkers | Controls (n=30) | Remitting-MS (n=30) | Relapsing-MS (n=30) | ||||
|---|---|---|---|---|---|---|---|
| Mean (SD) | Median (IQR) | Mean (SD) | Median (IQR) | Mean (SD) | Median (IQR) | P-value * | |
| PC | 99.13 (14.44) |
97.5 (25.25) |
110.66 (18.07) | 116.5 (28.5) |
116.86 (20.74) |
117 (30) |
0.001 a |
| aC4 | 5.78 (5.21) |
4.75 (1.725) |
2.75 (1.43) |
2.3 (1.125) |
3.91 (3.13) |
2.85 (2.107) | 0.001 b |
| aC9 | 3.38 (1.13) |
3.4 (1.675) |
4.91 (3.03) |
4.3 (3.3) |
5.42 (4.13) |
4.4 (3.54) |
0.019 c |
| AngI | 9.40 (4.04) |
8.38 (4.34) |
7.31 (2.96) |
7.26 (2.84) |
10.57 (4.83) |
9.76 (5.75) |
0.009 d |
| TF | 110.85 (56.31) | 120.69 (101.11) | 94.06 (55.83) |
99.23 (104.22) | 45.03 (37.66) |
22.72 (58.67) | <0.001 e |
| Tie-2 | 49.60 (17.58) |
43.15 (10.775) | 46.45 (17.36) |
40.85 (22.425) | 34.42 (19.94) |
35.7 (25.3) |
0.002 f |
| P-Sel | 245.85 (120.48) | 197 (156) | 216.26 (82.15) | 216.5 (131) |
169.41 (173.51) |
160 (215.4) | 0.020 g |
| VitA° | 40.75 (18.15) |
32.68 (21.925) | 43.15 (12.77) |
41.63 (12.43) |
105.18 (50.07) |
100.8 (101.13) | <0.001 h |
| VitK° | 163.89 (110.80) | 126.49 (119.56) | 166.75 (107.20) | 148.405 (167.115) | 295.51 (64.84) |
272.455 (299.222) | <0.001 i |
| B - Serological viral/microbiological assay | Controls (n=30) | Remitting-MS (n=30) | Relapsing-MS (n=30) | ||||
| Mean (SD) | Median (IQR) | Mean (SD) | Median (IQR) | Mean (SD) | Median (IQR) | P-value * | |
| EBV-EBNA IgG | 26.81 (7.48) | 27.65 (8.325) | 31.05 (6.35) |
32.15 (6.3) |
31.89 (4.66) |
32 (5.3) |
0.014 l |
| InfA IgA | 2.49 (3.23) | 1.45 (0.825) |
2.91 (2.72) |
2.1 (1.725) |
1.71 (1.23) |
1.35 (0.75) |
0.029 n |
| InfA IgG | 11.59 (7.61) | 8.75 (9.275) |
15.87 (7.49) |
15.7 (12.375) |
17.88 (11.98) |
12.45 (21.45) | 0.041 m |
| Sch IgG | 4.79 (1.74) | 4.6 (2.225) |
4.75 (2.28) |
4.15 (2.25) |
3.86 (1.41) |
3.4 (0.65) |
0.023° |
| C - Complete blood cell count | Controls (n=30) | Remitting-MS (n=30) | Relapsing-MS (n=30) | ||||
| Mean (SD) | Median (IQR) | Mean (SD) | Median (IQR) | Mean (SD) | Median (IQR) | P-value * | |
| MCHC | 33.11 (0.79) |
33.2 (0.875) |
32.94 (0.66) |
32.85 (0.775) |
33.45 (0.74) |
33.2 (1.25) |
0.027 p |
| Lym | 1.91 (0.55) |
1.82 (0.607) |
1.86 (0.57) |
1.84 (0.8) |
1.49 (0.60) |
1.48 (0.968) | 0.014 q |
| Neu/Lym | 1.85 (0.68) |
1.795 (0.995) | 2.24 (1.11) |
2.15 (1.08) |
3.42 (2.51) |
2.515 (2.23) | 0.005 r |
| Plt/Lym | 139.87 (43.82) | 132.345 (63.752) | 149.16 (66.38) | 143.23 (80.91) | 227.66 (2.2.92) | 173.545 (108.295) | 0.016 s |
| CD3+T | – | – | 1.43 (0.48) |
1.406 (0.764) |
1.11 (0.51) |
1.024 (0.528) | 0.015 |
| CD4+T | – | – | 0.92 (0.29) |
0.892 (0.43475) | 0.73 (0.38) |
0.653 (0.322) | 0.043 |
| CD8+T | – | – | 0.47 (0.25) |
0.405 (0.291) |
0.34 (0.18) |
0.336 (0.257) | 0.028 |
| CD19+B | – | – | 0.30 (0.15) |
0.275 (0.17) |
0.22 (0.13) |
0.223 (0.128) | 0.025 |
IQR, interquartile range; MS, multiple sclerosis; SD, standard deviation; for the abbreviations of all laboratory parameters, see Supplementary Table 1 .
°In the context of vitamin assessment, data were available in 57 subjects for Vit A and K (22 controls, 18 remitting- and 17 relapsing- MS patients).
*Kruskall-Wallis or ANOVA according to the variable distribution.
Significant differences are reported in bold; statistically significant post-hoc analysis details are described below:
P=0.042 for controls vs Remitting-MS; p=0.001 for controls vs Relapsing-MS.
P=0.002 for controls vs Remitting-MS; p=0.008 for controls vs Relapsing-MS; p=0.039 for Relapsing-MS vs Remitting-MS.
P=0.022 for controls vs Remitting-MS; p=0.017 for controls vs Relapsing-MS.
P=0.005 for Relapsing-MS vs Remitting-MS.
P<0.001 for Relapsing-MS vs controls; p<0.001 for Relapsing-MS vs Remitting-MS.
P<0.001 for Relapsing-MS vs controls; p=0.032 for Relapsing-MS vs Remitting-MS.
P=0.013 for Relapsing-MS vs controls; p=0.032 for Relapsing-MS vs Remitting-MS.
P<0.001 for Relapsing-MS vs controls; p<0.001 for Relapsing-MS vs Remitting-MS.
P<0.001 for Relapsing-MS vs controls; p<0.001 for Relapsing-MS vs Remitting-MS.
P=0.017 for controls vs Remitting-MS; p=0.011 for controls vs Relapsing-MS.
P=0.037 for controls vs Remitting-MS; p=0.037 for controls vs Relapsing-MS.
P=0.014 for Relapsing-MS vs Remitting-MS.
° P=0.014 for Relapsing-MS vs controls; p=0.038for Relapsing-MS vs Remitting-MS.
P=0.023 for Relapsing-MS vs Remitting-MS.
P=0.020 for Relapsing-MS vs controls; p=0.047 for Relapsing-MS vs Remitting-MS.
P=0.002 for Relapsing-MS vs controls; p=0.049 for Relapsing-MS vs Remitting-MS.
P=0.025 for Relapsing-MS vs controls C; p=0.026 for Relapsing-MS vs Remitting-MS.