Figure 4. The breadth of the anti-Cryptosporidium immune response was not correlated with protection from infection.

(A) Split violin plot of antibody breadth in plasma among the 100 most-reactive antigens (Y-axis) for each isotype (X-axis) is shown for the comparison between children that had no stool samples (diarrheal or surveillance) qPCR⁺ for Cryptosporidium parasites (purple) and children who had a verified Cryptosporidium infection (green). (B) Data is shown from one year old infants who had prior qPCR-confirmed Cryptosporidium infections (“Yr0-1 qPCR⁺”) that were subsequently uninfected (blue) or reinfected (orange) during the next 2 years. (C) Data is shown from 1-year-old infants that included both the immunologically naive infants with no prior Cryptosporidium infections detected by qPCR in stool samples (diarrheal or surveillance) as well as those with qPCR⁺ stool samples during the first year of life. Medians and quartiles are indicated by horizontal lines in each split violin. Significant P values from Wilcoxon’s rank sum tests are shown above violins.