Fig. 3.

Determination of the binding domains of cOSMRβ with cIL-31. a Schematic representation of EGFP-fused full-length or different truncates of cOSMRβ constructs used in this study. SP signal peptide, D1 domain 1, TM transmembrane domain, and CT cytoplasmic domain. b Binding ability of different cOSMRβ-EGFP-fusion proteins to rcIL-31 measured by flow cytometry. Biotinylated rcIL-31 at a concentration of 20.0 μg/ml was incubated with HEK293T cells, which were expressed with different cOSMRβ-EGFP-fusion proteins on the cell membrane. The binding signals were picked up by PE/Cyanine7-labeled streptavidin. The binding percentage was calculated based on the ratio of rcIL-31-Biotin- and EGFP-fusion protein double-positive cells to all EGFP-fusion protein-positive cells. The data were the statistical results of three independent experiments, and an error bar indicates the mean standard deviation (n = 6)