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. 2023 Aug 14;13:280. doi: 10.1038/s41398-023-02573-5

Fig. 2. Hspa12a−/− mice displayed impairment of neurogenesis, neuronal spinogenesis in hippocampus and displayed mood disorder.

Fig. 2

A Neuronal dendrite length and dendrite spine density. Neuronal dendrite length (Scale bar = 50 μm) in dentate gurus and spine density (Scale bar = 20 μm) of neuronal dendrite in dentate gurus was analyzed using Golgi staining. n = 6/group. B Adult hippocampal neurogenesis (AHN). AHN was examined in granular layer and interspace of dentate gyrus (DG) by BrdU incorporation. DAPI was used to counter stain nuclei. The images showed the representative staining in DG, and the boxed areas were magnified in the down panels. n = 5/group. Scale bar = 100 μm. C BDNF expression. The expression of BNDF was examined in hippocampus by immunoblotting. n = 8/group. D Sucrose preference test. The total liquid consumption and sucrose preference ratio were measured in 24 h duration in mice. E Open field test. The time spent, distance travelled, moving speed and entries in each area of an open field were recorded within 20 min duration. F Forced swimming test. Immobility episodes and time were recorded within 4 min duration of test. G. Tail suspension test. Immobility episodes and time were recorded within 4 min duration of test. H Elevated plus maze test. The time spent in open arm was recorded within 5 min of test. I Self-grooming test. The events of self-grooming were recorded within 10 min of test. Data are mean ± SD, **P < 0.01 and *P < 0.05 by Student’s two-tailed unpaired t test or Mann–Whitney U test test, n = 14 for WT group and n = 13 for Hspa12a−/− group.