Fig. 6. Building-associated changes in radial glial biology.

A Re-clustered radial glial subpopulations show building-associated B cyp19a1 expression (RG₃), C signatures of decreased quiescence (RG₂), and D increases in proportion (RG₄); n = 38 biologically independent animals (n = 19 building males, n = 19 control males). Gray lines link paired building/control males. UMAP plots in the right three panels represent effects in control (“C,” top) and building (“B,” bottom) males, pooled across individuals. In all box plots, the center line indicates the median, the bounds of the box indicate the upper and lower quartiles, and the whiskers indicate 1.5x interquartile range. Asterisks indicate significance at α = 0.05 after adjusting for a 5% false discovery rate. Source data are provided as a Source Data file, and additional related data can be found in Supplementary Data 7–9.