Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 15;9:85. doi: 10.1038/s41421-023-00573-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Dot blot assay showing the specificity of a pan anti-Ksu antibody. Control peptide, unmodified H3 N-terminal 1–21 aa; Ksu peptide, H3 1–21 aa with succinylated K9, K14, and K18; Kac peptide, H3 1–21 aa with acetylated K9, K14, and K18. The amounts of peptides in dot blot were as indicated. b–e WB analyses showing the levels of histone succinylation and acetylation in HeLa cells treated with an increasing dose of TSA for 12 h (b), 1 μM TSA for different periods (c), different inhibitors (d), and different concentrations of the class I HDAC inhibitor MS275 (e). f IF assay showing the different effects of TSA and NAM treatment on cellular succinylation and acetylation levels. HeLa cells were treated with 1 μM TSA or 20 mM NAM for 24 h. Scale bars, 20 μm. g IF staining showing the effect of MS275 treatment on cellular succinylation level. HeLa cells were treated with different concentrations of MS275 for 12 h. Scale bars, 20 μm.