Table 1.
MS analysis of in vitro histone desuccinylation by HDAC1.
| Sites | Peptide sequence | Counting number − HDAC1 | Counting number + HDAC1 |
|---|---|---|---|
| H2AK95 | R.NDEELNKsuLLGR.V | SC = 3 | SC = 0 |
| H3K18 | R.KsuQLATKAAR.K | SC = 3 | SC = 0 |
| H3K23 | R.KQLATKsuAAR.K | SC = 2 | SC = 0 |
| H3K37 | R.KSAPATGGVKKsuPHR.Y | SC = 3 | SC = 0 |
| H3K56 | R.YQKsuSTELLIR.K | SC = 2 | SC = 0 |
| H4K5 | R.GKsuGGKGLGKGGAKR.H | SC = 11 | SC = 0 |
| H4K8 | R.GKGGKsuGLGKGGAKR.H | SC = 17 | SC = 0 |
| H4K12 | R.GKGGKGLGKsuGGAKR.H | SC = 10 | SC = 0 |
| H4K16 | R.GKGGKGLGKGGAKsuR.H | SC = 7 | SC = 0 |
| H4K20 | R.KsuVLRDNIQGITKPAIR.R | SC = 4 | SC = 0 |
| H4K31 | R.DNIQGITKsuPAIR.R | SC = 13 | SC = 0 |
In vitro desuccinylation reactions were performed using histone substrates prepared from TSA-treated HeLa cells and without (−) or with (+) addition of immuno-affinity-purified HDAC1. The resulting histones were analyzed for succinylated histone peptides by LC-MS. “SC” represents spectrum counting number, which is semi-quantitative for peptide abundance.