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. 2023 Aug 14;9:56. doi: 10.1038/s41522-023-00420-5

Fig. 3. B. uniformis JCM5828 alters colonic transcriptome profile in mice with DSS-induced colitis.

Fig. 3

a Volcano plots for the RNA-Seq analyses of Con vs. Bu group. The red and green dots represent upregulated and downregulated DEGs in the Bu group, respectively. b The heatmap of DEGs expression in two groups. c The KEGG enrichment analysis of Con vs. Bu group. The genes behind each bar represent the gene involved in the pathway. The red and green genes represent upregulated and downregulated DEGs in the Bu group, respectively. d Bu binds to the IL-17 receptor (IL-17R) in colon epithelial cells and inhibits downstream NF-κB and MAPK signaling pathways through the signaling complex IL-17R-ACT1-TRAF6, thereby eliminating the transcription and expression of inflammatory factors in the nucleus. The blue box represents KEGG annotated differential genes downregulated in the Bu group. e, f Western blot analyses of p-P38/P38, p-JNK1/2/JNK1/2, p-ERK1/2/ERK1/2, p-P65/P65, p-IκB/IκB, and p-IKK/IKK in colon tissues using the respective anti-phospho-protein antibodies, and quantified phosphorylated protein and total protein density (n = 8). Data were analyzed using one-way ANOVA with Tukey’s test and expressed as the means ± SEM. *p < 0.05, ***p < 0.001.