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. 2022 Sep 16;39(4):1257–1274. doi: 10.1007/s10565-022-09744-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — GMNP_E-EV-miR-150-5p enhances the proliferation and maturation of osteoblasts. Osteoblasts were co-cultured with GMNP_E-EV-miR-150-5p mimic or GMNP_E-EV-miR-150-5p inhibitor. A Osteoblast viability measured by CCK-8 assay. B Alizarin red S staining of mineralized nodules in the osteoblasts. C ALP staining of ALP activity in the osteoblasts. D mRNA expression of RUNX2 and BSP in the osteoblasts determined by RT-qPCR. E Immunoblotting of RUNX2, BSP, OPN, and OCN proteins in the osteoblasts. * p < 0.05. The cell experiment was repeated 3 times independently