Fig. 4.

miR-150-5p strengthens the proliferation and maturation of osteoblasts by binding to MMP14. A Venn plot (http://bioinformatics.psb.ugent.be/webtools/Venn/) of downstream genes of miR-150-5p predicted by the miRBD website (http://mirdb.org/) and the osteogenesis-related genes predicted by the GeneCards website (relevance score ≥ 20; https://www.genecards.org/). B Binding sites between MMP14 and hsa-miR-150-5p as well as rno-miR-150-5p. C MMP14 mRNA expression in the bone tissues of normal and DO rats determined by RT-qPCR. D Immunoblotting of MMP14 protein in the bone tissues of normal and DO rats. n = 6. E Binding of miR-150-5p to MMP14 verified by dual-luciferase reporter assay in HEK-293 T cells. F miR-150-5p expression and MMP14 mRNA expression in osteoblasts in the presence of with miR-150-5p mimic alone or combined with oe-MMP14 determined by RT-qPCR. G Osteoblast viability in the presence of with miR-150-5p mimic alone or combined with oe-MMP14 measured by CCK-8 assay. H Alizarin red S staining of mineralized nodules in osteoblasts in the presence of with miR-150-5p mimic alone or combined with oe-MMP14. I ALP staining of ALP activity in osteoblasts. J mRNA expression of RUNX2 and BSP in osteoblasts in the presence of with miR-150-5p mimic alone or combined with oe-MMP14 determined by RT-qPCR. K Immunoblotting of RUNX2, BSP, OPN, and OCN proteins in osteoblasts in the presence of with miR-150-5p mimic alone or combined with oe-MMP14. * p < 0.05. The cell experiment was repeated 3 times independently