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. 2022 Sep 16;39(4):1257–1274. doi: 10.1007/s10565-022-09744-y

Fig. 6.

Fig. 6

GMNPE-EV-miR-150-5p enhances the osteogenesis of DO rats through the MMP14/Wnt/β-catenin axis. The DO rats were treated with GMNPE-EV-miR-150-5p mimic alone or combined with sh-β-catenin. A miR-150-5p expression and mRNA expression of MMP14 and β-catenin in the bone tissues of DO rats determined by RT-qPCR. B Immunoblotting of MMP14, Wnt1, and β-catenin proteins in the bone tissues of DO rats. C Immunohistochemistry analysis for the number of osteoblasts. D Immunofluorescence staining analysis (calcein double labeling) for the bone mineralization rate on the trabecular bone surface. E Serum levels of CTX-I and TRAP5b in DO rats determined by ELISA. F Micro-CT analysis of the BV/TV, Tb.Th, Tb.N, and Tb.Sp in the bone tissues (coronal section, sagittal section, and transverse section) of DO rats. * p < 0.05. n = 6