TABLE 4.

Identification of the minimal ori1 sequence

Plasmida	No. of transformantsb		Ratio (42°/30°C)
	30°C	42°C
pRB2	2,752	0	<3 × 10−4
pRB4	2,672	1,176	0.44
pGM614	2,776	0	<3 × 10−4
pGM615	2,204	0	<4 × 10−4
pGM628	26,500	13,160	0.49
pGM629	16,268	11,728	0.72
pGM632	9,960	7,304	0.73
pGM639	10,624	0	<9 × 10−5
pGM660	12,151	0	<8 × 10−5
pGM661	11,238	1	8 × 10−5
pGM675	13,147	11,952	0.90
pGM682	13,280	11,620	0.87
pGM683	10,292	8,240	0.80
pGM688	9,960	0	<1 × 10−4
pGM689	9,761	0	<1 × 10−4
pGM690	10,093	0	<1 × 10−4
pGM696	11,620	0	<8 × 10−5
pGM697	9,960	4,640	0.46
pGM698	9,960	0	<1 × 10−4
pGM699	9,628	2	2 × 10−4
pGM700	10,093	800	8 × 10−2
pGM704	15,936	13,088	0.82
pGM713	8,632	0	<1 × 10−4

^aThe plasmids are derivatives of pUC19 carrying different P4 fragments, as indicated in Materials and Methods. 

^bC-2107/pMK302 was transformed as described in Materials and Methods.