FIG. 2.

Localization of CDV products in the hypothalami of infected mice during the acute stage of infection. Viral mRNAs and proteins are expressed in a unique pattern in the hypothalamus of an infected Swiss mouse (14 days p.i.). (a) Immunohistochemical localization of cells containing viral proteins with serum from an SSPE patient. Strong labeling is mainly seen in the peri-third ventral hypothalamic areas, especially in hypothalamic nuclei, such as the VMH, DMH, and ARN, while outside the hypothalamus, structures such as the fornix (fx) are completely unlabeled. (b) Viral mRNAs, demonstrated by in situ hybridization with a dATP ³⁵S-3′-end-labeled NP oligonucleotide, are also found in hypothalamic nuclei, such as the VMH, DMH, and ARN. The distribution of labeling matched that for proteins, indicating active viral replication in the hypothalamus. (c) Viral NP gene mRNAs are also detected in neurons of the LH areas. The dotted lines represent schematic limits of the hypothalamic nuclei. Viii, third ventricle.