TABLE 3.

Attenuation of NP gene transcription in obese mice during the late (5 months p.i.) versus the acute (14 days p.i.) stage of infection^a

Structure	NP (late/acute) (%) in:
	Preobeseb	Obese
Hypothalamus	56	49
Hippocampus	39	23
Mesencephalon	44	22

^aRT-PCR was carried out on various microdissected structures from sham-inoculated and infected obese or nonobese mice. The RT-PCR schedule was 3 mM MgCl₂ and 30 cycles (95°C for 45 s, 60°C for 45 s, 72°C for 1 min). The results are expressed as counts per minute for late NP gene mRNAs/counts per minute for acute NP gene mRNAs (normalized to the housekeeping gene) × 100. The data indicate that the NP gene transcription level in brain structures of obese mice remained elevated (up to 50%) compared to that during active viral replication (14 days p.i.). 

^bAccording to leptinemia of 63 μg/liter (weight, 45 g).