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. 2023 Aug 10;37:101758. doi: 10.1016/j.tranon.2023.101758

Fig. 1.

Fig 1

Cancer-associated metabolic affects transcriptome. (A) Schematic representation of CRISPR/Cas9 strategy employed to generate PKM2 knockout breast cancer cell lines. (B) Real-time OCR in WT BBS and BBS Mut MCF7 cell lines. (C) Quantification of ΔOCR in the WT BBS and BBS Mut MCF7 cell lines. (D) Intracellular lactate production in WT BBS and BBS Mut MCF7 cell lines estimated by lactate assay. (E) Real-time ECAR analysis in WT BBS and BBS Mut MCF7 cell lines. (F) Quantification of ΔECAR in the WT BBS and BBS Mut MCF7 cell lines. (G) Volcano plot depicting DEGs in BBS Mut vs. WT BBS MCF7. (H) The top 20 enriched terms for biological processes for the DEGs obtained from the microarray data. (I) Violin plot depicting the differentially expressed epigenetic factors (blue points) and transcription factors (red points) in BBS Mut MCF7 cells. (J) Heat map representation of top differentially expressed transcription and epigenetic factors in the BBS Mut MCF7 cells. Data are represented as mean ± SD. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, ns= not significant.