Skip to main content
. 2023 Aug 10;37:101758. doi: 10.1016/j.tranon.2023.101758

Fig. 2.

Fig 2

c-Myc undergoes promoter-level histone lactylation. Immunoblot analysis of c-Myc in (A) WT BBS and BBS Mut MCF7 cells and (B) on subjecting BBS Mut MCF7 cells to 15 mM l-lactate treatment for 24 h. (C) Schematic representation of c-Myc promoter. The primers used to generate deletional constructs for luciferase assay are represented using black arrows. The primers used for ChIP qRT-PCR have been marked using red and blue arrows. (D) Luciferase assay performed using the deletional constructs of the c-Myc promoter depicting a dampened activity of −245 to +174 fragment in the BBS Mut MCF7 cells. (E) Immunoblot analysis of p300 in WT BBS and BBS Mut MCF7 cells. (F) H3K18la ChIP assay performed in WT BBS and BBS Mut MCF7 cells. (G) Immunoblot analysis of p300 upon subjecting BBS Mut MCF7 cells to 15 mM l-lactate treatment for 24 h. (H) H3K18la ChIP assay performed in BBS Mut MCF7 cells subjected to 15 mM l-lactate treatment for 12 h. Data are represented as mean± SD. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, ns= not significant.