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. 2023 Aug 10;37:101758. doi: 10.1016/j.tranon.2023.101758

Fig. 3.

Fig 3

c-Myc regulates SRSF10 expression. (A) c-Myc ChIP-seq analysis demonstrating its enrichment on SRSF10 promoter. Immunoblot analysis of c-Myc and SRSF10 upon overexpressing (B) PKM2 and (C) PFKFB3 in BBS Mut cell lines. (D) Immunoblot analysis demonstrating the effect of c-Myc knockdown on SRSF10 expression in WT BBS MCF7 cells. (E) Immunoblot assay performed upon overexpressing c-Myc in BBS Mut MCF7 cells. c-Myc ChIP assay performed in (F) WT BBS and BBS Mut MCF7 cells and (G) upon subjecting the BBS Mut MCF7 cells to 15 mM l-lactate treatment for 12 h. (H) Schematic representation of SRSF10 promoter luciferase construct depicting the wild-type c-Myc binding site (WTc-MycBS) and mutated c-Myc binding site (Mutc-MycBS). Luciferase assay of (I) WTc-MycBS SRSF10 construct performed in WT BBS and BBS Mut MCF7 cells and (J) WTc-MycBS and Mutc-MycBS SRSF10 constructs performed in WT BBS MCF7 cells. Data are represented as mean± SD. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, ns= not significant.