c-Myc-SRSF10 axis regulates alternative splicing of MDM4 and Bcl-x. (A) Predicted SRSF10 binding site on exon 9 of MDM4. (B) Predicted SRSF10 binding site on exon 2 of Bcl-x. (C) Schematic representation of MDM4 and Bcl-x splicing. (D) RPS16 normalized qRT-PCR analysis of MDM4-FL, Bcl-xL, and Bcl-xS in WT BBS and BBS Mut MCF7 cells. (E) RPS16 normalized qRT-PCR analysis of SRSF10, MDM4-FL, Bcl-xL, and Bcl-xS upon performing SRSF10 knockdown in WT BBS MCF7 cells. Data are represented as mean± SD. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, ns= not significant.