Figure 4.

Interacted CD36 proteins were screened using LC-MS/MS. (A) Mass spectrometry process flowchart. (B) Silver staining map of the co-IP assay with CD36 antibody in TCMK-1 cells. The first lane represents marker, the second lane represents co-IP using CD36 antibody, the third lane is IgG antibody, and the fourth lane is IP products of CD36 in TCMK-1 cells. (C) MS spectra for FSP1 fragment identified by LC-MS/MS. (D) Total protein samples from TCMK-1 cells were extracted and interacting CD36 proteins and FSP1 were detected via co-IP. Western blotting using anti-FSP1 and anti-CD36 antibodies showed that CD36 interacts with FSP1. (E) Representative immunofluorescence staining images of CD36 (green) and FSP1 (red) in TCMK-1 cells. (F) FSP1 expression after transfection with CD36 small interfering RNA (siRNA).