Table 1.
List of primers used in this study with references to original publications of the primers.
| Target | FW/RV | Sequence | Published in: * |
|---|---|---|---|
| mavs (1) | sgRNA | TGTCAGAAGGTGTAAGGCAA | |
| mavs (2) | sgRNA | CTGATGCTCCAACAGCTCCA | |
| mavs (3) | sgRNA | TTCCTTCTACCAGCTCTGAG | |
| irf3 | sgRNA | TTCTAGGAAGGATTGCTCCG | |
| irf7-1 | sgRNA | GCGAACAGATAAATAGTGGC | |
| mavs | FW | ACTGGACACCTAGGATCTCTGT | |
| RV | CAGCAACAGGAGAAGGTGCT | ||
| irf3 | FW | ACTGGCTGATAGAACAAGTG | |
| RV | ATGGGGGTCGTTTGAGTCCTTG | ||
| irf7-1 | FW | TCCCAGTTTACACAGGCTGTCA | |
| RV | GGTGCTTTACCTCCTGTGGGT | ||
| qPCR Target | FW/RV | Sequence | Published in: * |
| elf2a | FW | TGCCCCTCCAGGATGTCTAC | (33) |
| RV | CACGGCCCACAGGTACTG | ||
| ifna | FW | AAAACTGTTTGATGGGAATATGAAA | (29) |
| RV | CGTTTCAGTCTCCTCTCAGGTT | ||
| ifnc | FW | ATGTATGATGGGCAGTGTGG | (34) |
| RV | CCAGGCGCAGTAACTGAAAT | ||
| allmx | FW | TGCAACCACAGAGGCTTTGAA | (35) |
| RV | GGCTTGGTCAGGATGCCTAAT | ||
| ifit5 | FW | GCTGGGAAGAAGCTTAAGCAGAT | (21) |
| RV | TCAGAGGCCTCGCCAACT | ||
| SAV3 nsP1 | FW | CCGGCCCTGAACCAGTT | (36) |
| RV | GTAGCCAAGTGGGAGAAAGCT | ||
| elf2a | FW | TGCCCCTCCAGGATGTCTAC | (33) |
| RV | CACGGCCCACAGGTACTG | ||
| Probe | AAATAGGCGGTATTGG | ||
| ifna (ifna1-2) | FW | TGACTGGATCCGACACCACT | |
| RV | ATCTCCTCCCATCTGGTCCA | ||
| Probe | AGCGCAGAATACCTTTCCCT | ||
| ifnc (ifnc1-4) | FW | ATACCGCCAGATTGAAGAGAG | |
| RV | CAGTCCTTCTGTCCTGATGAGATA | ||
| Probe | GGGCAGTGTGGATACCAGTG | ||
| mavs | FW | GCTGATGAACTGAGGGCAGA | |
| RV | GGTAGCAGCAGGTGAAGGAG | ||
| Probe | AGCACAACCAGAACAATCCCT | ||
| irf3 | FW | CAGGATTCCTGCAGCGATGA | |
| RV | GTCGCCTTGAACCCTACCAT | ||
| Probe | ATTTTCAAGGCGTGGGCTGA | ||
| irf7 | FW | CTCCGAGGACGACCGTAAAA | |
| RV | CCTTGTCAGTGGGATGCTCA | ||
| Probe | TATTCAGGGCATGGGCAGTG |
*If no reference is given, the primers were designed specifically for this investigation.