FIG. 2.
Inhibition of EMCV replication in cultured mouse PM treated with NO inhibitors. (A) Peritoneal macrophages (106/well) were seeded in 24-well cluster plates as described in Materials and Methods. After a 24-h incubation and a vigorous washing, PM cultures were infected with EMCV at an MOI of 1. After viral adsorption, incubation proceeded for 48 h in the presence of various concentrations of AA, and virus yields were then determined as described in Materials and Methods. Values represent the mean of three different experiments, and each experiment contained duplicate samples per condition. Statistical analysis by Kruskall-Wallis test gave the following results: 0 versus 500 μM AA, P < 0.006; 0 versus 1,000 μM AA, P < 0.004. (B) Experiment was performed as for panel A in the presence of 30 μM carboxy-PTIO. Values represent a mean of three different experiments, and each experiment contained duplicate samples per condition. A statistical analysis by Kruskall-Wallis test gave the following result: carboxy-PTIO versus control, P < 0.02. (C) Experiment was performed as for panel A in the presence of 1,000 μM AA at different times of in vitro culture before infection (0, 1, or 6 days).