FIG. 3.
Inhibition of EMCV replication in L929 cells cocultured with NMMA-treated PM. PM were previously cultivated in medium alone for 24 h. L929 cells, infected with EMCV at an MOI of 10−5, were then added to PM cultures as described in Materials and Methods at a macrophage/target cell ratio of 1. Virus yields were determined after 24 h of infection in the presence of 100 μM NMMA. Statistical analysis by Kruskall-Wallis test gave the following results: L-EMCV versus L-EMCV + PM, P < 0.05; L-EMCV + NMMA versus L-EMCV + PM + NMMA, P < 0.05; L-EMCV + PM versus L-EMCV + PM + NMMA, P < 0.05.