Table 2.

Viral vectors for CRISPR/Cas9 delivery system.

Delivery	Packaging capacity	Advantages	Disadvantages
Lentivirus	Approximately 10 kb	High transduction efficiency; large cargo size; low immunogenicity; ability to transduce dividing and non-dividing cells in different tissues	Non-specific DNA integration causes cancer risk; complex packaging structure
Adenovirus	Approximately 8–10 kb	Efficient delivery; large cargo size	Inflammatory response
Adeno-associated virus	Approximately 4.7 kb	Multiple serotypes; low immunogenicity; ability to transduce dividing and non-dividing cells in different tissues	Pre-existing neutralizing antibodies; long-term expression of Cas9 causes off-target effects