Chemicals
Reagent or resource	Source	Identifier
Mitotracker Green FM	Molecular Probes, Invitrogen	M7514
TMRE	Molecular Probes, Invitrogen	T669
Mitotracker Red CM‐H2X ROS	Molecular Probes, Invitrogen	M7513
Cycloheximide	Sigma Aldrich	C7698
Mitotracker Deep Red FM	Molecular Probes, Invitrogen	M22426
Paraquat	Sigma Aldrich	856177
CCCP	Sigma‐Aldrich	857815
Magnetic Beads	Merc, Millipore	LSKMAGAG02
Levamisole	Sigma‐Aldrich	196142
RNAse inhibitor	Roche	3335399001
Nanobeads 100 nm	Polysciences	6401015
DMSO	AppliChem	A3672,0250
Glycoblue	ThermoFisher Scientific	AM9515
CIP enzyme	NEB	M0290
Completemini Proteinase inhibitor coctail	Roche	11836153001
Protector RNase Inhibitor	Roche	3335399001
Zirconium Oxide Beads 0.5 mm	Next Advance
DiO6(3)	Invitrogen	D275
BioTracker ATP‐Red live cell dye	Merck	SCT045
Antibodies
Type	Source	Identifier
Anti‐GFP	Minotech	‐
Anti‐MTCOI	Abcam	ab14705
Anti‐alpha tubulin	DSHB	AA4.3 (concentrate)
JLA20 (anti‐actin)	DHSB	‐
Anti‐ATP5A	Abcam	ab14748
Anti‐MRPL13	ThermoFisher Scientific	PA5‐51007
Anti‐NDUFS8	abbexa	abx026882
Anti‐RFP	Minotech	‐
Experimental models
Organism/Strain	Source
C. elegans: Strain N2: Wild Type	Caenorhabditis Genetics Center
C. elegans: Strain SJ4103: N2;Is[p myo‐3 mtGFP]	Caenorhabditis Genetics Center
C. elegans: Strain SJ4143: N2;Is[p ges‐1 mtGFP]	Caenorhabditis Genetics Center
C. elegans: Strain CL2166: N2;Is[p gst‐4 GFP]	Caenorhabditis Genetics Center
C. elegans: Strain OP168: unc‐119(ed3) III;[skn‐1::TY1::EGFP::3xFLAG;unc‐119(+)]	Caenorhabditis Genetics Center
C. elegans: Strain AGD383: uthIs202 [aak‐2 (intron 1)::aak‐2(aa1‐aa321)::Tomato::unc‐54 3′UTR;pRF4]	Caenorhabditis Genetics Center
C. elegans: Strain CB1370: daf‐2(e1370)III	Caenorhabditis Genetics Center
C. elegans: Strain MQ887: isp‐1(qm150)	Caenorhabditis Genetics Center
C. elegans: Strain TJ1052: age‐1(hx546)	Caenorhabditis Genetics Center
C. elegans: Strain RB759: akt‐1(ok525)	Caenorhabditis Genetics Center
C. elegans: Strain MQ133: nuo‐6(qm200)	Caenorhabditis Genetics Center
C. elegans: Strain TK22: mev‐1(kn1)III	Caenorhabditis Genetics Center
C. elegans: Strain IR1284: N2;Is[p myo‐3 mtGFP];Ex011[p lgg‐1 DsRed::LGG‐1]	Palikaras et al (2015b)
C. elegans: Strain SPC167: skn‐1(lax120)IV;P gst‐4 GFP	Caenorhabditis Genetics Center
C. elegans: daf‐2(e1370);Is[p gst‐4 GFP]	This paper
C. elegans: N2;EX[p edc‐3 EDC‐3::DsRed;pRF4]	Rieckher et al (2018)
C. elegans: unc‐119 (ed3)III;EX[p ntl‐2 NTL‐2::GFP;unc‐119(+)]	This paper
C. elegans: unc‐119 (ed3)III;Ex[p edc‐3 EDC‐3::DsRed;p ntl‐2 NTL‐2::GFP;unc‐119(+)]	This paper
C. elegans: unc‐119 (ed3)III;EX[p dcap‐2 DCAP‐2::mcherry;unc‐119(+)]	This paper
C. elegans: unc‐119 (ed3)III;Ex[p ccf‐1 CCF‐1::GFP;unc‐119(+)]	This paper
C. elegans: N2;Ex[p ife‐2 GFP;pRF4]	Syntichaki et al (2007)
C. elegans: unc‐119 (ed3)III;Ex[p ntl‐2 NTL‐2::GFP;unc‐119(+)];syEx1155 [p myo‐3 TOMM‐20::mRFP::3xMyc;unc‐119(+)]	This paper
C. elegans: SJ4103; N2;Ex[p edc‐3 EDC‐3::DsRed; pRF4]	This paper
C. elegans: unc‐119 (ed3)III;Ex[p edc‐3 EDC‐3::GFP;unc‐119(+)]	This paper
C. elegans: unc‐119 (ed3)III;Ex003[p myo‐3 TOMM‐20::Rosella;unc‐119(+)]	This paper
C. elegans: Strain PS6187: syEx1155[p myo‐3 TOMM‐20::mRFP::3xMyc;unc‐119(+)]	Caenorhabditis Genetics Center
C. elegans: Strain JJ1850: unc‐119(ed3)III; Is[his‐72(1kb 5′UTR)::his‐72::SRPVAT::GFP::his72 (1KB 3′UTR)]	Caenorhabditis Genetics Center
C. elegans: Strain VC682: ntl‐2(ok974)/mIn1 [mIs14 dpy‐10(e128)]II	Caenorhabditis Genetics Center
C. elegans: Strain N2;Ex[ pdcap‐1 DCAP‐1::DsRed;pRF4]	Rieckher et al (2018)
C. elegans: Strain SJZ328: foxSi75[eft‐3p::tomm‐20::mKate2::HA::tbb‐2 3′UTR]I	Caenorhabditis Genetics Center
C. elegans: Strain unc‐119(ed3)III;Ex[p ccf‐1 CCF‐1::GFP;p dcap‐2 DCAP‐2::mCherry;unc‐119(+)]	This paper
C. elegans: Strain N2;Ex[p ntl‐2 NTL‐2::GFP;p dcap‐2 DCAP‐2::mCherry;pRF4]	This paper
C. elegans: Strain unc‐119(ed3)III;Ex[p edc‐3 EDC‐3::GFP;p dcap‐2 DCAP‐2::mCherry;unc‐119(+)]	This paper
C. elegans: Strain unc‐119(ed3)III;Ex[p f46b6.6 F46B6.6::GFP;unc‐119(+)]	This paper
Oligonucleotides
Name	Sequence
Primer: ntl‐2 promoter Forward	ACACGACGGATCATTTCACGAG
Primer: ntl‐2 promoter Reverse	GGATCCCTGAAAGAAAATCGATTT
Primer: ntl‐2 coding Forward	GGATCCATTTATGAGTAGAACGTAGCCAT
Primer: ntl‐2 coding Reverese	ACCGGTGGGTTTGGAGTAGCTCG
Primer: dcap‐2 promoter Forward	ACATTGCACTATACCCCTCTTATTGC
Primer: dcap‐2 promoter Reverse	ACGAGAGATTCAAGCAAGTGGTGTC
Primer: dcap‐2 coding part 1 Forward	GCGGCCGCATGCAGCAACA
Primer: dcap‐2 coding part 1 Reverse	TCTAGACGTTTCAGCGAGTAATGAAACTTTTG
Primer: dcap‐2 coding part 2 Forward	TCTAGAGATAGTGAAGCCCTTTCTCTTTT
Primer: dcap‐2 coding part 2 Reverse	CCCGGGTGGTAATTGTGGTC
Primer: ccf‐1 promoter Forward	TTCGTGTTTTGAAGAATTATCTTGTAAAATGAG
Primer: ccf‐1 promoter Reverse	AAGCTTCTAGAATTTTCGTTTTAGAGTGAACG
Primer: ccf‐1 coding Forward	TTCCAGATATCATAAAAATGGCTTCTAGTAGC
Primer: ccf‐1 coding Reverse	ACCGGTGGGGCTTGTTGTGGAACT
Primer: f46b6.6 promoter Forward	AGTTATCATTACAGTTTCGCTGGTTTTATATATGAAAC
Primer: f46b6.6 promoter Reverse	GCTAGCCTGGAACTTAATTATGCAACGAT
Primer: f46b6.6 coding Forward	ATGTCCAAAACGATGATTCTACAGTTGTTG
Primer: f46b6.6 coding Reverse	ACCGGTAAATCCTGGTGGATACCAGT
Primer: ntl‐2 RNAi Forward	ATGTTAGCAGACGACCATCAAGTCG
Primer: ntl‐2 RNAi Reverse	TTAGTTTGGAGTAGCTCGCGCC
Primer: atp‐3 RNAi Forward	AGAACAAGCTCGACCAGATTTC
Primer: atp‐3 RNAi Reverse	GGGCATCCTTGTATTTCTTGAC
Primer: mrps‐5 RNAi Forward	ATGGCATCACTTTTGCCATTTGTC
Primer: mrps‐5 RNAi Reverse	ACCGGTCTTTTTGGGAACCACATGCGA
Primer: tomm‐20 RNAi Forward	ATGTCGGACACAATTCTTGGTTTCAAC
Primer: tomm‐20 RNAi Reverse	CTCCAAGTCGTCGGTGTCATCGA
Primer: akap‐1 RNAi Forward	ACCGGTGAACATTTTCTCACTTACTG
Primer: akap‐1 RNAi Reverse	TTTGCGACGAGAATGATGGTC
Primer: xrn‐1 RNAi Forward	GATGAATTCACAAGTGGAAAAAGCTCTGAC
Primer: xrn‐1 RNAi Reverse	GATGAATTCGATGAAGCCGTCGGA
Primer: nhr‐49 RNAi Forward	CATCTGAATCACATCCACGATC
Primer: nhr‐49 RNAi Reverse	ATTATTCTGCTCACTGTTCAAAATG
Primer: Mito1 Forward	GTTTATGCTGCTGTAGCGTG
Primer: Mito1 Reverse	CTGTTAAAGCAAGTGGACGAG
Primer: ama‐1 Forward	TGGAACTCTGGAGTCACACC
Primer: ama‐1 Reverse	CATCCTCCTTCATTGAACGG
Primer: skn‐1 Forward	TCCACCAGGATCTCCATTCG
Primer: skn‐1 Reverse	CTCCATAGCACATCAATCAAGTCG
Primer: aak‐2 Forward	CAGAGCGCATTGCAGCGTC
Primer: aak‐2 Reverse	GTTGAGCACCTTCCACTCCATGTC
Primer: atp‐5 Forward	ACTGGTCGAAGCTCGCCGAG
Primer: atp‐5 Reverse	ACTCGGCTGGAACCTCTCCG
Primer: f46b6.6 Forward	GTTTGGAAGCAAATGAATCCTTCAGA
Primer: f46b6.6 Reverse	CTGCAAAATCGAAACATCGTCAAGT
Primer: h28o16.1 Forward	ACCTTGAGGAGACCGGAAAGGTT
Primer: h28o16.1 Reverse	TTCCGAAGACGACAACTCCGA
Primer: mrpl‐13 Forward	GTGATTGATGCGAATCAACAAGAC
Primer: mrpl‐13 Reverse	CATATTGAACTTATAAATTGTGTGTTTCCA
Primer: mrps‐5 Forward	AGAAAATTCTCGAAGAACGAGATACTGGA
Primer: mrps‐5 Reverse	ACAATAAGTTTCAAAATCATCGAAATTCACA
Primer: nuo‐5 Forward	CGGCTCCACCGAAAAAGGTC
Primer: nuo‐5 Reverse	TCACCGACTTCTCCACCTCTACCA
Primer: spcs‐1 Forward	GTCGCCGAGAGAACTTACCAGGTAA
Primer: spcs‐1 Reverse	ACAATCGGGTTCTTTCTGAAGAGGA
Primer: t20h4.5 Forward	GACCACTCAGTTCAAGATTCAGAGG
Primer: t20h4.5 Reverse	ATAATCCACAATAGATGCACTTGGTC
Primer: pmp‐3 Forward	ATGATAAATCAGCGTCCCGAC
Primer: pmp‐3 Reverse	TTGCAACGAGAGCAACTGAAC
Primer: fib‐1 Forward	TCGTTGGACCAGAAGGAATCGTT
Primer: fib‐1 Reverse	AAAGATGACATCGACCATTCCGA
Primer: npp‐22 Forward	ACTCAATTTTTCGCATATCCAAGTGAAT
Primer: npp‐22 Reverse	ACCATGCTGAGAATCCAGAAACCAT
Primer: rhi‐1 Forward	GAGCTTCTCAATGCTGACAAGGAGG
Primer: rhi‐1 Reverse	GGAACCTTCTTTGATGGAAACAGAC