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. 2023 Jul 31;10:1200317. doi: 10.3389/fmed.2023.1200317

Figure 3.

Figure 3

Comparison between WT and KO colon, ileum and urinary proteomes and relative expression of Abcb1a in the KO model. (A) Abcb1a knockout gave rise to the major source of variation in the colon proteome, resulting in clustering of mice corresponding to their genotype (KO vs. WT mice) in the sPCA model. A similar trend was observed in the ileum and urine proteomes, but the effect of Abcb1a knockout did not give rise to as much variation in herein to discriminate between WT and KO mice as evidenced by overlap between 95% confidence ellipses. (B, C) Functional enrichment of colon proteins explaining the difference between WT and KO were associated with immune-related processes, transport of organic substances, neutrophil degranulation and cellular stress. Across proteome comparison of enriched biological processes, we identify processes related to metabolism and immune-associated processes. (D) Validation of expression of Abcb1a transporter in the colon of WT and Abcb1a KO mice (***: Fisher’s exact test p-value < 0.001). (KO Knockout; WT Wildtype).