Table 1.
Type, incidence, and severity scores of histopathological changes in colons of Abcb1a knockout groups treated with either casein (CTL, control) or red meat-based diets (MEAT).
| Parameter | Knockout CTL | Knockout MEAT | ||||
|---|---|---|---|---|---|---|
| Sacrifice | After 5 weeks | After 10 weeks | After 20 weeks | After 5 weeks | After 10 weeks | After 20 weeks |
| Thickening of colonic mucosa | 2/2a | 6/6 | 4/4 | 2/2 | 5/6 | 5/5b |
| Changes in epithelium | ||||||
| Score Ec | ||||||
| Mean | 1.5 | 1.8 | 1.3 | 2.0 | 1.2 | 1.4 |
| Range | 1–2 | 1–2 | 1–2 | 2–2 | 0–2 | 0–2 |
| Inflammatory cell infiltration of colon | ||||||
| Score Id | ||||||
| Mean | 3.5 | 2.3 | 2.8 | 3.0 | 1.7 | 2.6 |
| Range | 3–4 | 2–3 | 2–3 | 3–3 | 0–2 | 2–3 |
| Total histologic score (mean E + mean I) | 5.0 | 4.1 | 4.1 | 5.0 | 2.9 | 4.0 |
| Mucosal erosion or ulceration | 0/2 | 2/6 | 1/4 | 0/2 | 0/6 | 0/5 |
| Ulcer | 0/2 | 0/6 | 0/4 | 0/2 | 0/6 | 2/5 |
| Cryptic abscess or cryptitis | 1/2 | 2/6 | 2/4 | 2/2 | 3/6 | 5/5 |
aIncidence of a change is expressed by the number of colons with a given change of total number examined. bColon from one Knockout MEAT animal was not available for microscopic examination (Supplementary Table S2). cScore system from Obermeier et al. (32) with modifications: 0: normal morphology; 1: loss of goblet cells; 2: loss of goblet cells in large area (in several folds); 3: loss of crypts; 4: loss of crypts in large area. dScore system from Obermeier et al. (32) with modifications: 0: no infiltrate; 1: infiltrate around crypt basis; 2: infiltrate reaching muscularis mucosa; 3, loss of crypts; 4: infiltration of submucosa, tunica muscularis and serosa. (KO, Knockout; WT, Wildtype).