Figure 2.

(a) NH₃, NO₂^–, and NO₃^– content of patches (6.0 cm × 6.0 cm) of latex and nitrile gloves cut from the center of the each glove. The N content was determined by cutting the patches into smaller chunks and sonicating them in 30 mL of 0.1 M KOH solution for 15 min. A Celgard membrane (2.5 cm × 2.5 cm) was exposed by rubbing the top and bottom surfaces with a nitrile glove. (b) The NH₃, NO₂^–, and NO₃^– content of cell materials was determined by sonicating 2.5 cm × 2.5 cm samples, except for the carbon paper and Cu electrodeposited on carbon paper (Cu EL), which were 1.2 cm diameter discs, in a 0.1 M KOH solution for 15 min. NH₃ detection was done by the spectrophotometric indophenol blue method. Both NO₂^– and NO₃^– were quantified by ion chromatography. *NO₂^– assay was performed with the spectrophotometric Griess test due to Cl^– overlap in the ion chromatogram. The error bars indicate the standard deviation of three individual experiments.