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. 2023 Aug 11;14(1):20220271. doi: 10.1515/tnsci-2022-0271

Figure 4.

Figure 4

Knockdown of circYIPF6 inhibits proliferation and glycolysis and promotes apoptosis of glioma cells via sponging miR-760. (a) qRT-PCR confirmation of the miR-760 expression in miR-760 inhibitor-transfected glioma cells (n = 3). (b) EdU staining of glioma cells transfected with sh-NC + anti-miR-NC, sh-circYIPF6 + anti-miR-NC or sh-circYIPF6 + anti-miR-760, and EdU-positive cell rate (n = 3). (c) Colonies formed in glioma cells transfected with sh-NC + anti-miR-NC, sh-circYIPF6 + anti-miR-NC, or sh-circYIPF6 + anti-miR-760 (n = 3). (d and e) Flow cytometry detection of cell cycle distribution of glioma cells after sh-NC + anti-miR-NC, sh-circYIPF6 + anti-miR-NC, or sh-circYIPF6 + anti-miR-760 transfection (n = 3). (f) Flow cytometry detection of cell apoptosis of glioma cells after sh-NC + anti-miR-NC, sh-circYIPF6 + anti-miR-NC, or sh-circYIPF6 + anti-miR-760 transfection (n = 3). (g) Glucose uptake and (h) lactate concentration were measured in sh-NC + anti-miR-NC-, sh-circYIPF6 + anti-miR-NC-, or sh-circYIPF6 + anti-miR-760-transfected glioma cells (n = 3). (i and j) Western blot displaying the expression of Bax, Bcl2, GLUT1, and LDHA in glioma cells transfected with sh-NC + anti-miR-NC, sh-circYIPF6 + anti-miR-NC, or sh-circYIPF6 + anti-miR-760 (n = 3). ***P < 0.001, **P < 0.01.