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. 2023 Aug 11;14(1):20220271. doi: 10.1515/tnsci-2022-0271

Figure 5.

Figure 5

circYIPF6 regulates the expression of PTBP1 through sponging miR-760. (a) The predicted miR-760 binding sites in the 3′UTR of PTBP1 mRNA. (b and c) Luciferase reporter activity of PTBP1 3′UTR-WT or PTBP1 3′UTR-MUT in glioma cells after transfection with miR-760 (n = 3). (d) mRNA expression of PTBP1 in tumor tissues (n = 57) and normal tissues (n = 43) were assayed by qRT-PCR. (e) The protein level of PTBP1 in tumor tissues (n = 57) and normal tissues (n = 43) was analyzed by western blot. (f) The protein level of PTBP1 in cells was examined by western blot (n = 3). (g) Western blot analysis of PTBP1 protein expression after miR-760 mimics or miRNA mimics transfection (n = 3). (h) Western blot analysis of PTBP1 protein expression after sh-NC, sh-circYIPF6, sh-circYIPF6 + anti-miR-NC, or sh-circYIPF6 + anti-miR-760 transfection (n = 3). ***P < 0.001, **P < 0.01.