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. 2023 Aug 11;14(1):20220271. doi: 10.1515/tnsci-2022-0271

Figure 6.

Figure 6

circYIPF6 silencing inhibits proliferation and glycolysis and facilitates apoptosis of glioma cells through downregulating PTBP1. (a) Western blot for the protein expression of PTBP1 in glioma cells transfected with pcDNA-NC or pcDNA-PTBP1 (n = 3). (b) EdU staining of glioma cells transfected with sh-NC + pcDNA-NC, sh-circYIPF6 + pcDNA-NC or sh-circYIPF6 + pcDNA-PTBP1, and EdU-positive cell rate (n = 3). (c) Colonies formed in glioma cells transfected with sh-NC + pcDNA-NC, sh-circYIPF6 + pcDNA-NC, or sh-circYIPF6 + pcDNA-PTBP1 (n = 3). (d and e) Flow cytometry detection of cell cycle distribution of glioma cells after sh-NC + pcDNA-NC, sh-circYIPF6 + pcDNA-NC, or sh-circYIPF6 + pcDNA-PTBP1 transfection (n = 3). (f) Flow cytometry detection of cell apoptosis of glioma cells after sh-NC + pcDNA-NC, sh-circYIPF6 + pcDNA-NC, or sh-circYIPF6 + pcDNA-PTBP1 transfection (n = 3). (g) Glucose uptake and (h) lactate concentration were measured in sh-NC + pcDNA-NC-, sh-circYIPF6 + pcDNA-NC-, or sh-circYIPF6 + pcDNA-PTBP1-transfected glioma cells (n = 3). (i and j) Western blot displaying the expression of Bax, Bcl2, GLUT1, and LDHA in glioma cells transfected with sh-NC + pcDNA-NC, sh-circYIPF6 + pcDNA-NC, or sh-circYIPF6 + pcDNA-PTBP1 (n = 3). ***P < 0.001, **P < 0.01.