Figure 1.

Cytotoxicity of 22-(4′-pyridinecarbonyl) jorunnamycin A (22-(4′-py)-JA) and jorunnamycin A (JA) in the H460 and A549 cells. (A) Chemical structures of 22-(4′-py)-JA and JA. (B) H460 and (C) A549 cells were treated with various concentrations of 22-(4′-py)-JA or JA for 24, 48, and 72 h and cell viability was measured using the MTT assay and presented as a percentage of the cell viability. (D) IC₅₀ value of 22-(4′-py)-JA and JA in H460 and A549 cells. (E) H460 and (F) A549 cells were incubated with nontoxic concentrations of 22-(4′-py)-JA for 24 and 48 h and cell proliferation was examined using the MTT assay and presented as a percentage of cell proliferation. Data are presented as the mean ± SEM (n = 3). *p < 0.05 vs untreated control cells. All data were analyzed by using analysis of variance (ANOVA) followed by the Tukey post hoc test.